Publications

Copy number aberrations (CNAs) are critical drivers of genomic diversity in oncology, where recurrent CNAs frequently underlie tumorigenesis. However, existing public resources are limited in their somatic CNA specificity, breadth across multiple data modalities, and support for recurrent CNAs with online functional annotation and interactive visualization. Here, we present CNAScope (https://cna.compbio.com/), a database that curates and functionally annotates over 3,954,361 CNA profiles and 3,946,319 metadata from 810 datasets, 174,464 samples, 3,018,672 single cells, and 764,232 spatial cells/spots, spanning 77 cancer subtypes from eight data sources and 55 cancer initiatives and institutions. CNAScope offers downloadable CNA annotations and interactive visualizations at bin, gene, and pathway term levels, including phylogenetic inference, clustering, dimension reduction, and focal/consensus CNA detection. Users can explore data through interactive heatmaps, phylogenetic trees, embedding plots, CN charts, and focal/consensus plots, or upload and annotate their own CNAs in real time. In all, with its large curated data volume and rich annotation capabilities, CNAScope serves as a vital resource for accelerating cancer research.

Abstract Schizophrenia (SCZ) is characterized by synaptic structural deficits, yet how dysregulated noncoding RNAs (ncRNAs) drive these abnormalities remains unknown. Through integrative multilayered analysis of SCZ data from whole transcriptome sequencing (blood samples), GWAS risk loci, and expression data using pipeline ceRNAxis, the THUMPD3-AS1/miR-485-5p/ARHGAP8 axis is identified as a key regulator of synaptic function. Functional validation reveals that THUMPD3-AS1 acts as a competitive endogenous RNA, sequestering miR-485-5p and thereby derepressing ARHGAP8. Despite suppressing RhoA activity, ARHGAP8 enhances ROCK2 activation through RhoB/C-mediated compensatory mechanisms. Hyperactivation of ROCK2 through this noncanonical pathway disrupted actin cytoskeletal remodeling patterns, leading to increased immature dendritic spines and synaptic ultrastructural defects, which are pathological features associated with SCZ. In vivo, ventral hippocampal (vHip) overexpression of miR-485-5p or targeted knockdown of THUMPD3-AS1 rescued MK-801-induced SCZ-like phenotypes (anxiety, cognitive deficits, and social memory impairments) and restored synaptic ultrastructure. Crucially, this regulatory axis is cross-species conservation, with bidirectional expression changes validated in patient-derived blood and vHip tissues of mice. The findings reveal a novel ncRNA-driven pathogenic cascade in SCZ, where dysregulated RhoB/C-ROCK2 signaling, distinct from classical RhoA pathways, mediates synaptic destabilization. This presents a therapeutic axis for precision interventions targeting noncanonical actin cytoskeletal remodeling.